論文 - 建石 寿枝
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New Modified Deoxythymine with Dibranched Tetraethylene Glycol Stabilizes G-Quadruplex Structures 査読あり 国際誌
Hisae Tateishi-Karimata, Tatsuya Ohyama, Takahiro Muraoka, Shigenori Tanaka, Kazushi Kinbara, Naoki Sugimoto
MOLECULES 25 ( 3 ) 2020年2月
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出版者・発行元：MDPI
Methods for stabilizing G-quadruplex formation is a promising therapeutic approach for cancer treatment and other biomedical applications because stable G-quadruplexes efficiently inhibit biological reactions. Oligo and polyethylene glycols are promising biocompatible compounds, and we have shown that linear oligoethylene glycols can stabilize G-quadruplexes. Here, we developed a new modified deoxythymine with dibranched or tribranched tetraethylene glycol (TEG) and incorporated these TEG-modified deoxythymines into a loop region that forms an antiparallel G-quadruplex. We analyzed the stability of the modified G-quadruplexes, and the results showed that the tribranched TEG destabilized G-quadruplexes through entropic contributions, likely through steric hindrance. Interestingly, the dibranched TEG modification increased G-quadruplex stability relative to the unmodified DNA structures due to favorable enthalpic contributions. Molecular dynamics calculations suggested that dibranched TEG interacts with the G-quadruplex through hydrogen bonding and CH-pi interactions. Moreover, these branched TEG-modified deoxythymine protected the DNA oligonucleotides from degradation by various nucleases in human serum. By taking advantage of the unique interactions between DNA and branched TEG, advanced DNA materials can be developed that affect the regulation of DNA structure.
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Chemical biology of non-canonical structures of nucleic acids for therapeutic applications 査読あり 国際誌
Hisae Tateishi-Karimata, Naoki Sugimoto
CHEMICAL COMMUNICATIONS 56 ( 16 ) 2379 - 2390 2020年2月
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出版者・発行元：ROYAL SOC CHEMISTRY
DNA forms not only the canonical duplex structure but also non-canonical structures. Most potential sequences that induce the formation of non-canonical structures are present in disease-related genes. Interestingly, biological reactions are inhibited or dysregulated by non-canonical structure formation in disease-related genes. To control biological reactions, methods for inducing the formation of non-canonical structures have been developed using small molecules and oligonucleotides. In this feature article, we review biological reactions such as replication, transcription, and reverse transcription controlled by non-canonical DNA structures formed by disease-related genes. Furthermore, we discuss recent studies aimed at developing methods for regulating these biological reactions using drugs targeting the DNA structure.
DOI： 10.1039/c9cc09771f
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Effect of potassium concentration on triplex stability under molecular crowding conditions 査読あり 国際共著
Y. Teng, H. Tateishi-Karimata, T. Ohyama, and N. Sugimoto
Molecules 25 387 2020年1月
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Effect of Potassium Concentration on Triplex Stability under Molecular Crowding Conditions 査読あり 国際共著 国際誌
Ye Teng, Hisae Tateishi-Karimata, Tatsuya Ohyama, Naoki Sugimoto
MOLECULES 25 ( 2 ) 2020年1月
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出版者・発行元：MDPI
The properties of non-canonical DNA structures, like G-quadruplexes and triplexes, change under cell-mimicking molecular crowding conditions relative to dilute aqueous solutions. The analysis of environmental effects on their stability is crucial since they play important roles in gene expression and regulation. In this study, three intramolecular and intermolecular triplex-forming sequences of different C(*)(+)G-C triplet content ((*): Hoogsteen base pair; - : Watson-Crick base pair) were designed and their stability measured in the absence and presence of a crowding agent with different K+ concentrations. In dilute solution, the stability of the triplexes was reduced by decreasing the concentration of KCl. This reduction became smaller as the number of C(*)(+)G-C triplets increased. Under molecular crowding conditions, Watson-Crick base pairs and Hoogsteen base pairs were destabilized and stabilized, respectively. Interestingly, with lower KCl concentrations (<= 1 M), the destabilization of the triplexes due to reduction of KCl concentration was significantly smaller than in dilute solutions. In addition, the C(*)(+)G-C content had greater influence on triplex stability under molecular crowding conditions. Our work provides quantitative information about the effects of K+ concentration on triplex stability under molecular crowding conditions and should further our understanding of the function and regulation of triplexes in bioprocesses.
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Effect of chemical environment changes in cell on DNA structures and functions 招待あり
Hisae Tateishi-Karimata and Naoki Sugimoto
Impact 2020 ( 7 ) 25 - 27 2020年
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出版者・発行元：Science Impact Ltd
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Validation of the nearest-neighbor model for Watson-Crick self-complementary DNA duplexes in molecular crowding condition. 査読あり
Ghosh S, Takahashi S, Endoh T, Tateishi-Karimata H, Hazra S, Sugimoto N
Nucleic acids research 47 ( 7 ) 3284 - 3294 2019年4月
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C-rich sequence in a non-template DNA strand regulates structure change of G-quadruplex in a template strand during transcription 査読あり
建石 寿枝
Bull. Chem. Soc. Jpn. 92 572 - 577 2019年
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A Turn-On Detection of DNA Sequences by Means of Fluorescence of DNA-Templated Silver Nanoclusters via Unique Interactions of a Hydrated Ionic Liquid. 査読あり
Teng Y, Tateishi-Karimata H, Tsuruoka T, Sugimoto N
Molecules (Basel, Switzerland) 23 ( 11 ) 2889 2018年11月
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Mirror-Image Dependence: Targeting Enantiomeric G-Quadruplex DNA Using Triplex Metallohelices. 査読あり 国際共著
Zhao C, Song H, Scott P, Zhao A, Tateishi-Karimata H, Sugimoto N, Ren J, Qu X
Angewandte Chemie (International ed. in English) 57 ( 48 ) 15723 - 15727 2018年10月
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An anionic phthalocyanine decreases NRAS expression by breaking down its RNA G-quadruplex 査読あり
Kawauchi Keiko, Sugimoto Wataru, Yasui Takatoshi, Murata Kohei, Itoh Katsuhiko, Takagi Kazuki, Tsuruoka Takaaki, Akamatsu Kensuke, Tateishi-Karimata Hisae, Sugimoto Naoki, Miyoshi Daisuke
NATURE COMMUNICATIONS 9 ( 1 ) 2271 2018年6月
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Biological and nanotechnological applications using interactions between ionic liquids and nucleic acids. 査読あり
Tateishi-Karimata H, Sugimoto N
Biophysical reviews 10 ( 3 ) 931 - 940 2018年6月
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Pursuing origins of (poly)ethylene glycol-induced G-quadruplex structural modulations 査読あり 国際共著
Trajkovski Marko, Endoh Tamaki, Tateishi-Karimata Hisae, Ohyama Tatsuya, Tanaka Shigenori, Plavec Janez, Sugimoto Naoki
NUCLEIC ACIDS RESEARCH 46 ( 8 ) 4301 - 4315 2018年5月
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Alkylating probes for the G-quadruplex structure and evaluation of the properties of the alkylated G-quadruplex DNA 査読あり
Sato Norihiro, Takahashi Shuntaro, Tateishi-Karimata Hisae, Hazemi Madoka E., Chikuni Tomoko, Onizuka Kazumitsu, Sugimoto Naoki, Nagatsugi Fumi
ORGANIC & BIOMOLECULAR CHEMISTRY 16 ( 9 ) 1436 - 1441 2018年3月
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Drastic stability change of X-X mismatch in d(CXG) trinucleotide repeat disorders under molecular crowding condition 査読あり
Teng Ye, Pramanik Smritimoy, Tateishi-Karimata Hisae, Ohyama Tatsuya, Sugimoto Naoki
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 496 ( 2 ) 601 - 607 2018年2月
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テトラエチレングリコールで修飾されたグアニン四重鎖の安定性の分子動力学計算による解析
大山達也, 建石寿枝, 田中成典, 杉本直己
日本核酸化学会誌 2 3 - 10 2018年
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Unexpected Position-Dependent Effects of Ribose G-Quartets in G-Quadruplexes 査読あり 国際共著
Zhou Jun, Amrane Samir, Rosu Frederic, Salgado Gilmar F., Bian Yunqiang, Tateishi-Karimata Hisae, Largy Eric, Korkut Dursun Nizam, Bourdoncle Anne, Miyoshi Daisuke, Zhang Jian, Ju Huangxian, Wang Wei, Sugimoto Naoki, Gabelica Valerie, Mergny Jean-Louis
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 139 ( 23 ) 7768 - 7779 2017年6月
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Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers 査読あり 国際共著
Pica Andrea, Krauss Irene Russo, Parente Valeria, Tateishi-Karimata Hisae, Nagatoishi Satoru, Tsumoto Kouhei, Sugimoto Naoki, Sica Filomena
NUCLEIC ACIDS RESEARCH 45 ( 1 ) 461 - 469 2017年1月
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Local thermodynamics of the water molecules around single- and double-stranded DNA studied by grid inhomogeneous solvation theory 査読あり
Nakano Miki, Tateishi-Karimata Hisae, Tanaka Shigenori, Tama Florence, Miyashita Osamu, Nakano Shu-ichi, Sugimoto Naoki
CHEMICAL PHYSICS LETTERS 660 250 - 255 2016年9月
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G-Quadruplexes with Tetra(ethylene glycol)-Modified Deoxythymidines are Resistant to Nucleases and Inhibit HIV-1 Reverse Transcriptase 査読あり
Tateishi-Karimata Hisae, Muraoka Takahiro, Kinbara Kazushi, Sugimoto Naoki
CHEMBIOCHEM 17 ( 15 ) 1399 - 1402 2016年8月
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Expansion of the DNA Alphabet beyond Natural DNA Recognition. 査読あり
Tateishi-Karimata H, Sugimoto N
Chembiochem : a European journal of chemical biology 17 ( 14 ) 1301 - 1303 2016年7月