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Position |
Associate Professor |
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Research Field |
Life Science / Functional biochemistry, Nanotechnology/Materials / Bio chemistry |
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External Link |
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Graduating School 【 display / non-display 】
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Konan University Faculty of Science Graduated
- 2003.3
Graduate School 【 display / non-display 】
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Konan University Graduate School, Division of Science and Technology Doctor's Course Completed
- 2008.3
Campus Career 【 display / non-display 】
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Associate Professor
2020.4
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Lecturer
2016.4 - 2020.3
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KONAN UNIVERSITY Frontier of Institute for Biomolecular Engineering Research in Science and Technology Department of Nanobiochemistry Assistant Professor
2010.7 - 2016.3
External Career 【 display / non-display 】
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株式会社ファイン
2008.4 - 2009.2
Country：Japan
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米国イリノイ大学
2008.4 - 2008.6
Country：United States
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日本学術振興会
2005.4 - 2008.3
Country：Japan
Professional Memberships 【 display / non-display 】
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Japan Society of Nucleic Acids Chemistry
2019
Research Career 【 display / non-display 】
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Development of new DNA materials using ionic liquids
(not selected)
Project Year： 2010.1 -
Papers 【 display / non-display 】
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In-Cell Stability Prediction of RNA/DNA Hybrid Duplexes for Designing Oligonucleotides Aimed at Therapeutics. International journal
Dipanwita Banerjee, Hisae Tateishi-Karimata, Maria Toplishek, Tatsuya Ohyama, Saptarshi Ghosh, Shuntaro Takahashi, Marko Trajkovski, Janez Plavec, Naoki Sugimoto
Journal of the American Chemical Society 145 ( 43 ) 23503 - 23518 2023.11
In cells, the formation of RNA/DNA hybrid duplexes regulates gene expression and modification. The environment inside cellular organelles is heterogeneously crowded with high concentrations of biomolecules that affect the structure and stability of RNA/DNA hybrid duplexes. However, the detailed environmental effects remain unclear. Therefore, the mechanistic details of the effect of such molecular crowding were investigated at the molecular level by using thermodynamic and nuclear magnetic resonance analyses, revealing structure-dependent destabilization of the duplexes under crowded conditions. The transition from B- to A-like hybrid duplexes due to a change in conformation of the DNA strand guided by purine-pyrimidine asymmetry significantly increased the hydration number, which resulted in greater destabilization by the addition of cosolutes. By quantifying the individual contributions of environmental factors and the bulk structure of the duplex, we developed a set of parameters that predict the stability of hybrid duplexes with conformational dissimilarities under diverse crowding conditions. A comparison of the effects of environmental conditions in living cells and in vitro crowded solutions on hybrid duplex formation using the Förster resonance energy transfer technique established the applicability of our parameters to living cells. Moreover, our derived parameters can be used to estimate the efficiency of transcriptional inhibition, genome editing, and silencing techniques in cells. This supports the usefulness of our parameters for the visualization of cellular mechanisms of gene expression and the development of nucleic acid-based therapeutics targeting different cells.
DOI： 10.1021/jacs.3c06706
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Nearest-neighbor parameters for the prediction of RNA duplex stability in diverse in vitro and cellular-like crowding conditions. Reviewed International journal
Saptarshi Ghosh, Shuntaro Takahashi, Dipanwita Banerjee, Tatsuya Ohyama, Tamaki Endoh, Hisae Tateishi-Karimata, Naoki Sugimoto
Nucleic acids research 51 ( 9 ) 4101 - 4111 2023.5
RNA performs various spatiotemporal functions in living cells. As the solution environments significantly affect the stability of RNA duplexes, a stability prediction of the RNA duplexes in diverse crowded conditions is required to understand and modulate gene expression in heterogeneously crowded intracellular conditions. Herein, we determined the nearest-neighbor (NN) parameters for RNA duplex formation when subjected to crowding conditions with an ionic concentration relevant to that found in cells. Determination of the individual contributions of excluded volume effect and water activity to each of the NN parameters in crowded environments enabled prediction of the thermodynamic parameters and their melting temperatures for plenty of tested RNA duplex formation in vitro and in cell with significant accuracy. The parameters reported herein will help predicting RNA duplex stability in different crowded environments, which will lead to an improved understanding of the stability-function relationship for RNAs in various cellular organelles with different molecular environments.
DOI： 10.1093/nar/gkad020
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Simple and fast screening for structure-selective G-quadruplex ligands Reviewed
59 4891 - 4894 2023.4
DOI： 10.1039/D3CC00556A
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High-temperature adaptation of an OsNRT2.3 allele is thermoregulated by small RNAs. Reviewed International coauthorship International journal
Yong Zhang, Hisae Tateishi-Karimata, Tamaki Endoh, Qiongli Jin, Kexin Li, Xiaoru Fan, Yingjun Ma, Limin Gao, Haiyan Lu, Zhiye Wang, Art E Cho, Xuefeng Yao, Chunming Liu, Naoki Sugimoto, Shiwei Guo, Xiangdong Fu, Qirong Shen, Guohua Xu, Luis Rafael Herrera-Estrella, Xiaorong Fan
Science advances 8 ( 47 ) eadc9785 2022.11
Climate change negatively affects crop yield, which hinders efforts to reach agricultural sustainability and food security. Here, we show that a previously unidentified allele of the nitrate transporter gene OsNRT2.3 is required to maintain high yield and high nitrogen use efficiency under high temperatures. We demonstrate that this tolerance to high temperatures in rice accessions harboring the HTNE-2 (high temperature resistant and nitrogen efficient-2) alleles from enhanced translation of the OsNRT2.3b mRNA isoform and the decreased abundance of a unique small RNA (sNRT2.3-1) derived from the 5' untranslated region of OsNRT2.3. sNRT2.3-1 binds to the OsNRT2.3a mRNA in a temperature-dependent manner. Our findings reveal that allelic variation in the 5' untranslated region of OsNRT2.3 leads to an increase in OsNRT2.3b protein levels and higher yield during high-temperature stress. Our results also provide a breeding strategy to produce rice varieties with higher grain yield and lower N fertilizer input suitable for a sustainable agriculture that is resilient against climate change.
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DNA methylation is regulated by both the stability and topology of G-quadruplex. Reviewed International journal
Saki Matsumoto, Hisae Tateishi-Karimata, Naoki Sugimoto
Chemical communications (Cambridge, England) 58 ( 89 ) 12459 - 12462 2022.11
Since DNA methylation alters the chromatin state and regulates gene expression, elucidating the regulatory mechanisms of DNA methylation in response to environmental changes in the cell is crucial and urgent in understanding and regulating DNA methylation. G-quadruplex (G4) regulates transcription, translation and replication. Although it has recently been suggested that G4 regulates methylation, the detailed regulatory mechanism remains unclear. Here, we systematically analysed the effect of G4 formation on DNA methylation using G4s with various stabilities and topologies. The methylation efficiency decreased as the stability of G4 increased. Moreover, the degree of methylation suppression can be also controlled by G4 topology. Furthermore, our results showed the possibility of regulating methylation by modulating G4 stability and topology.
DOI： 10.1039/d2cc04383a
Books and Other Publications 【 display / non-display 】
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月刊化学「化学者が思い描く未来の社会」
建石 寿枝（ Role： Joint author , ⑥非二重らせん核酸により生命の機能を制御する ―生物種を超えた統一ルール）
2021.12
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CSJカレントレビュー「進化を続ける核酸化学―ゲノム編集，非二重らせん，核酸医薬」
神谷 真子, 建石 寿枝, 永 次史, 山吉 麻子, 杉本 直己（ Role： Joint author , 第1章フロントランナーに聞く「令和の時代も進化を続ける核酸化学」）
2021.10
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相分離生物学の全貌（現代化学増刊46)
建石寿枝, 杉本直己（ Role： Joint author , 第Ⅳ部 生物学的相分離の理論）
東京化学同人 2020.11 （ ISBN:9784807913466 ）
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生体分子化学―基礎から応用まで (エキスパート応用化学テキストシリーズ)
杉本 直己, 内藤 昌信, 橋詰 峰雄, 高橋 俊太郎, 田中 直毅, 建石 寿枝, 遠藤 玉樹, 津本 浩平, 長門石 曉, 松原 輝彦, 上田 実, 朝山 章一郎
講談社 2017.1 （ ISBN:406156806X ）
Review Papers (Misc) 【 display / non-display 】
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A Novel G4 Ligand Screening System with G4-selective Fluorescent Probe
橋本佳樹, 取井猛流, 藤田ひな, 川内敬子, 建石寿枝, 杉本直己, 三好大輔
日本化学会春季年会講演予稿集(Web) 102nd 2022
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The function of rDNA G-quadruplex aggregates formed by nucleolar stress
杉本渉, 桑本慎, 建石寿枝, 岩根敦子, 岩根敦子, 岩根敦子, 高橋宏隆, 三好大輔, 川内敬子
日本薬学会年会要旨集(Web) 141年会 29V05 - am10S 2021.3
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Molecular mechanism of the G-quadruplex formation for ribosomal DNA
取井猛流, 杉本渉, 建石寿枝, 鶴田充生, 月生雅也, 村嶋貴之, 西方敬人, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 44th 2021
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Analysis of liquid-liquid phase separation phenomenon by interaction between rDNA-derived DNA G4 and RGG peptide
月生雅也, 取井猛流, 建石寿枝, 西方敬人, 杉本直己, 三好大輔, 川内敬子
日本分子生物学会年会プログラム・要旨集(Web) 44th 2021
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Nucleic Acids Chemistry beyond the Watson-Crick Double Helix (69): Analysis of interactions between RNA G-quadruplexes and dipeptide repeats related to neurodegenerative diseases
大山達也, 建石寿枝, 田中成典, 杉本直己, 杉本直己
日本化学会春季年会講演予稿集(Web) 101st 2021
Presentations 【 display / non-display 】
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「核酸の姿と病気の関わり」 Invited
建石 寿枝
日本薬学会関西支部主催：市民公開講座「環境によって変わる核酸の姿と病気：ヒトからウイルスまでを標的とした創薬を目指して」 2022.12
Event date： 2022.12
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生物種を超えた多元応答機構の解明を目指した核酸構造の解析
建石寿枝
第95回日本生化学会大会 シンポジム「非二重らせん核酸の多元機能」 2022.11
Event date： 2022.11
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Quantitative analysis for G-quadruplex and i-motif formations in malignant cancers
Hisae Tateishi-Karimata, Naoki Sugimoto
2022.11
Event date： 2022.11
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細胞夾雑模倣系の構築と細胞内活性分子の設計指針
建石寿枝, 三好大輔, 杉本直己
分子夾雑の生命化学成果とりまとめ公開シンポジム 2022.9
Event date： 2022.9
Industrial property rights 【 display / non-display 】
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核酸の立体構造を制御する方法及びその用途、並びに、細胞内分子クラウディング環境を再現するための組成物
建石 寿枝、高橋 俊太郎、川内 敬子、杉本 直己
Application no：2022-189538
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核酸塩基対の安定性を塩基対選択的に変える方法
建石 寿枝、 杉本 直己
Application no：特願2011-117381
Country of applicant：Domestic
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佐々木 義晴, 西田 尚広, 瀧上 忠一, 建石 寿枝
Academic Awards Received 【 display / non-display 】
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日本化学会第94春季年会 優秀講演賞（学術）
2014.3 日本化学会
建石寿枝
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5th International Symposium on Nucleic Acids Chemistry ポスター優秀賞 (Nucleic Acids Research賞)
2007.11 ａ
狩俣 寿枝 （建石の旧姓 ）
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日本化学会第87春季年会 学生講演賞
2007.11 ａ
狩俣 寿枝 （建石の旧姓 ）
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第18回生命分子化学研究会若手サマースクール ポスター賞
2006.11 ａ
狩俣 寿枝 （建石の旧姓 ）
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Quantitative prediction of nucleic acid structures and functions affected by spaciotemporal environmental factors in cells
2022.4 - 2027.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research(S)
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Elucidation of the mechanism for dimensional response genome using intracellular environmental evaluation systems and development of dimensional response genome bank
2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
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Elucidation of the mechanism for dimensional response genome
2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
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2021.8 - 2024.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid (B) (Tentative)
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Do ion channels control the formation of non-canonical nucleic acids and gene expression?
2020.7 - 2022.3
JSPS Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)
Sugimoto Naoki
The formation of nucleic acids of non-canonical structures induces mutations in biological reactions such as replication, transcription, and translation. In diseased cells, the molecular environments, especially ionic environments, are very different from that of normal cells due to overexpression (or inactivation) of disease-specific ion channels. These environmental differences are thought to affect the formation of non-canonical structures. In this study, we analyzed nucleic acid structures in the environments that mimic the molecular environment in diseased cells using physicochemical methods. As results, we clarified the mechanisms of stability changes of the non-canonical structures by intracellular environmental factors. Furthermore, we developed techniques to control gene expression by ion-nucleic acid interactions using small molecules and modified nucleic acids.
Other External funds procured 【 display / non-display 】
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極限環境により誘起されるDNA特殊構造を活用したDNAスイッチの開発
2014.4 - 2015.3
公益財団法人ひょうご科学技術協会 公益財団法人ひょうご科学技術協会 平成26年度学術研究助成
Preferred joint research theme 【 display / non-display 】
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機能性核酸および酵素の活性を溶液環境で制御することを活用したナノマテリアル（センサーなど）の開発
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細胞内で活用できる機能性核酸の開発
Social Activities 【 display / non-display 】
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なでしこscientistトーク
Role(s)： Appearance, Presenter
甲南大学 先端生命工学研究所 なでしこscientistトーク 甲南大学先端生命工学研究所 2014.6
Audience： High school students, College students, Graduate students, Teachers, Guardians, Researchesrs, General
最先端の科学技術について、女性研究者がわかりやすく解説する講演会。
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第10回なでしこscientistトーク「新型コロナウィルス感染症に挑む（COVID-19）」
Role(s)： Lecturer
甲南大学先端生命工学研究所 親和女子高等学校 2020.10
Academic Activities 【 display / non-display 】
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第95回日本生化学会大会のシンポジウム 2S09e 「非二重らせん核酸の多元機能」
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
日本生化学会 ・ 今西 未来（京都大学化学研究所)・建石 寿枝（甲南大学先端生命工学研究所(FIBER)) ・学術変革領域研究(B)「多元応答ゲノム」 （ 名古屋国際会議場 第9会場（222） ） 2022.11
Type：Competition, symposium, etc.
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B領域横断研究会（糖化学ノックイン・多元応答ゲノム）
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
学術変革領域（B）「糖化学ノックイン」「多元応答ゲノム」 （ グランフロント大阪北館 アクティブスタジオ ） 2022.10
Type：Competition, symposium, etc.
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ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～ＫＡＫＥＮＨＩ
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 （ 甲南大学ポートアイランドキャンパス ） 2022.8
遺伝子を観て、新しい機能について学ぼう～mRNAワクチンやPCR検査のしくみ～
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ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～ＫＡＫＥＮＨＩ
Role(s)： Planning, management, etc., Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 （ 甲南大学ポートアイランドキャンパス ） 2022.8
体験しよう PCR 検査！学ぼう遺伝子の仕組み！
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FIBER核酸化学ユニバース９
Role(s)： Panel moderator, session chair, etc.
甲南大学先端生命工学研究所 学術変革領域（B）「多元応答ゲノム」多元応答ゲノム領域推進センター （ オンライン ） 2022.2
Type：Competition, symposium, etc.
Qualification acquired 【 display / non-display 】
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High School Teacher Specialization License