論文 - 遠藤 玉樹
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Photo inducible RNA interference using cell permeable protein carrier
Endoh T, Sisido M, Ohtsuki T
Nucleic Acids Symp. Ser., 51 127 - 128 2007年11月
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担当区分：筆頭著者
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Construction of intramolecular luciferase complementation probe for detecting specific RNA 査読あり
Endoh Tamaki, Mie Masayasu, Funabashi Hisakage, Sawasaki Tatsuya, Endo Yaeta, Kobatake Eiry
BIOCONJUGATE CHEMISTRY 18 ( 3 ) 956 - 962 2007年5月
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Construction of intramolecular luciferase complementation probe for detecting specific RNA 査読あり
Endoh T, Mie M, Funabashi H, Sawasaki T, Endo Y, Kobatake E
Bioconjug. Chem., 18 956 - 962 2007年3月
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担当区分：筆頭著者
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Tamaki Endoh, Hisakage Funabashi, Masayasu Mie, Eiry Kobatake
Analytical Chemistry 77 ( 14 ) 4308 - 4314 2005年7月
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Detection of specific nucleic acids is important to understand cellular mechanisms and functions of gene regulation. Here, we demonstrated a novel method to detect specific nucleic acids using recombinant protein and oligonucleotides. A recombinant protein YRGnC-11ad, which has a Rev-peptide between enhanced yellow fluorescent protein (EYFP) and enhanced cyan fluorescent protein (ECFP) was constructed and expressed in HeLa cells. Rev-peptide, which corresponds to amino acids 34-50 of the HIV-1 Rev protein, indicates disordered structure in solution but forms α-helical and elongated conformation upon binding to Rev response element RNA (RRE-RNA) and Rev-aptamer, respectively. We confirmed that YRGnC-11ad could specifically bind to RRE-RNA and Rev-aptamer in cell lysate, and fluorescent resonance energy transfer (FRET) signal was changed upon binding following the conformational change of Rev-peptide. To utilize this FRET signal change toward the detection of specific nucleic acids, we split the RRE-RNA sequence and connected to the complementary oligonucleotide for target nucleic acids. When each two oligonucleotides hybridized to an adjacent region of target nucleic acids correctly, a Rev-peptide binding site was reformed on the hybridized complex. And we could confirm that YRGnC-11ad recombinant protein indicated FRET increase upon binding to the hybridized complex in cell lysate. These results suggest that the recombinant protein probe is available for specific nucleic acid detection. © 2005 American Chemical Society.
DOI： 10.1021/ac048491j
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Novel method for detection of specific nucleic acids by recombinant protein with FRET 査読あり
Endoh T, Funabashi H, Mie M, Kobatake E
Anal. Chem., 77 4308 - 4314 2005年7月
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担当区分：筆頭著者
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Induction of neural differentiation by electrically stimulated gene expression of NeuroD2. 査読あり
Mie M, Endoh T, Yanagida Y, Kobatake E, Aizawa M
J. Biotechnol., 100 231 - 238 2003年2月
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