論文 - 臼井 健二
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Peptide-DNA Hybrid G-Quadruplex Structures for Regulation of Protein Expression Depending on Protease Activity 査読あり
A. Okada, M. Taniguchi, K. Usui, N. Sugimoto
Peptides 2013 180 - 181 2013年12月
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担当区分:筆頭著者
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Systematic screening of the cellular uptake of designed alpha-helix peptides 査読あり
Kenji Usui, Takuya Kikuchi, Masayasu Mie, Eiry Kobatake, Hisakazu Mihara
BIOORGANIC & MEDICINAL CHEMISTRY 21 ( 9 ) 2560 - 2567 2013年5月
出版者・発行元:PERGAMON-ELSEVIER SCIENCE LTD
The cellular penetration (CP) activity of functional molecules has attracted significant attention as one of the most promising new approaches for drug delivery. In particular, cell-penetrating peptides (CPPs) have been studied extensively in cellular engineering. Because there have been few large-scale systematic studies to identify peptide sequences with optimal CP activity or that are suitable for further applications in cell engineering, such as cell-specific penetration and cell-selective culture, we screened and compared the cellular uptake (CU) activity of 54 systematically designed a-helical peptides in HeLa cells. Furthermore, the CU activity of 24 designed peptides was examined in four cell lines using a cell fingerprinting technique and statistical approaches. The CU activities in various cells depended on amino acid residues of peptide sequences as well as charge, a-helical content and hydrophobicity of the peptides. Notably, the mutation of a single residue significantly altered the CU ability of a peptide, highlighting the variability of cell uptake mechanisms. Moreover, these results demonstrated the feasibility of cell-selective culture by conducting cell-selective permeation and death in cultures containing two cell types. These studies may lead to further peptide library design and screening for new classes of CPPs with useful functions. (C) 2013 Elsevier Ltd. All rights reserved.
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T. Kakiyama, K. Usui, K.-y. Tomizaki, M. Mie, E. Kobatake, H. Mihara
Polym. J. 45 ( 5 ) 525 - 539 2013年5月
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Construction of Artificial Peptides for Control of DNA G-quadruplex Structures Depending on Protease Activity toward Regulation of Protein Expression 査読あり
Arisa Okada, Kenji Usui, Mai Taniguchi, Keita Kobayashi, Naoki Sugimoto
Peptide Science 2012 285 - 286 2013年3月
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担当区分:筆頭著者
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A PNA Peptide for the Control of Site-Specific Silica Precipitation on DNA 査読あり
Kenji Usui, Kazuma Nagai, Hiroto Nishiyama, Takaaki Tsuruoka, Satoshi Fujii
Peptide Science 2012 375 - 376 2013年3月
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担当区分:筆頭著者
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A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release 査読あり
Kenji Usui, Takuya Kikuchi, Kin-ya Tomizaki, Takashi Kakiyama, Hisakazu Mihara
CHEMICAL COMMUNICATIONS 49 ( 57 ) 6394 - 6396 2013年
出版者・発行元:ROYAL SOC CHEMISTRY
We developed a novel peptide array format incorporating a photo-cleavable linker for monitoring cellular uptake. Model peptides were successfully immobilised via the photo-cleavable linker onto conventional plates and could be released spatiotemporally using UV irradiation. Incorporation of confocal microscopy allowed for detailed real-time monitoring of cellular internalisation of peptides.
DOI: 10.1039/c3cc41632a
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Screening of Designed Peptides for Binders to a DNA G-Quadruplex Structure 査読あり
N. Matsui, K. Kobayashi, K. Usui
Peptide Science 2011 2011 315 - 316 2012年3月
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担当区分:筆頭著者
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De Novo Designed Nitric Oxide Sensor Protein consisted of Iron Complex-Peptide Conjugate 査読あり
H. Miyazaki, K. Usui, S. Fujii
Peptide Science 2011 2011 303 - 304 2012年3月
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Development of PNA-Peptides Controlling DNA G-Quadruplex Structures Depending on Protease Activity 査読あり
K. Usui, K. Kobayashi, N. Sugimoto
Peptide Science 2011 2012 315 - 316 2012年3月
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担当区分:筆頭著者
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Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity. 査読あり
Usui K, Kakiyama T, Tomizaki KY, Mie M, Kobatake E, Mihara H
Bioorganic & medicinal chemistry letters 21 ( 21 ) 6281 - 6284 2011年11月
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Thermodynamic stability of Hoogsteen and Watson-Crick base pairs in the presence of histone H3-mimicking peptide 査読あり 国際共著
Smritimoy Pramanik, Kaori Nakamura, Kenji Usui, Shu-ichi Nakano, Sarika Saxena, Jun Matsui, Daisuke Miyoshi, Naoki Sugimoto
CHEMICAL COMMUNICATIONS 47 ( 10 ) 2790 - 2792 2011年
出版者・発行元:ROYAL SOC CHEMISTRY
We found that Hoogsteen base pairs were stabilized by molecular crowding and a histone H3-mimicking peptide, which was not observed for Watson-Crick base pairs. Our findings demonstrate that the type of DNA base pair is critical for the interaction between DNA and histones.
DOI: 10.1039/c0cc05776b
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Use of a designed peptide library to screen for binders to a particular DNA G-quadruplex sequence 査読あり
Keita Kobayashi, Noriko Matsui, Kenji Usui
Journal of Nucleic Acids 2011 572873 2011年
We demonstrated a method to screen for binders to a particular G-quadruplex sequence using easily designed short peptides consisting of naturally occurring amino acids and mining of binding data using statistical methods such as hierarchical clustering analysis (HCA). Despite the small size of the library used in this study, candidates of specific binders were identified. In addition, a selected peptide stabilized the G-quadruplex structure of a DNA oligonucleotide derived from the promoter region of the protooncogene c-MYC. This study illustrates how a peptide library can be designed and presents a screening guideline for construction of G-quadruplex binders. Such G-quadruplex peptide binders could be functionally modified to enable switching, cellular penetration, and organelle-targeting for cell and tissue engineering. © 2011 Keita Kobayashi et al.
DOI: 10.4061/2011/572873
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PNA-Peptide Conjugates for Regulation of DNA and RNA G-Quadruplex Structures Depending on a Particular Protease Concentration 査読あり
Kenji Usui, Keita Kobayashi, Naoki Sugimoto
Peptides 2010 2010 608 - 609 2011年
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担当区分:筆頭著者
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Nanomolar aggregation of amyloid-beta peptides covalently and site-specifically modified by cholesterol oxidation products 査読あり 国際共著
Kenji Usui, Evan T. Powers, Johan F. Paulsson, Sarah J. Siegel & Jeffery W. Kelly
Peptides 2008 2008 588 - 589 2011年
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担当区分:筆頭著者
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Fluorescent and Luminescent Fusion Proteins for Detection of Amyloid Beta Peptide Localization and Aggregation 査読あり
Kenji Usui, Masayasu Mie, Takashi Andou, Naoki Sugimoto, Hisakazu Mihara, Eiry Kobatake
Peptides 2010 2010 488 - 489 2011年
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担当区分:筆頭著者
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Designed Peptide Libraries for Protein and Cell analyses 査読あり
K. Kikuchi, K. Usui, K.-Y. Tomizaki, T. Takahashi, H. Mihara
Peptide Science 2010 2010 21 - 21 2011年
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Nanomolar Aggregation and Neurotoxicity of Amyloid beta Peptides with Site-Specific Modification of Oxidized Cholesterol 査読あり 国際共著
K. Usui, J. D. Hulleman, J. F. Paulsson, S. J. Siegel, E. T. Powers, J. W. Kelly
Peptide Science 2010 2010 169 - 169 2011年
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担当区分:筆頭著者
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A PNA Peptide Inducing DNAs to Form G-Quadruplex Structures Depending on a Protease Activity 査読あり
Kenji Usui, Keita Kobayashi, Naoki Sugimoto
Peptides 2011 2011 152 - 153 2011年
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担当区分:筆頭著者
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A Novel Nitric Oxide Sensor Using Fluorescent Peptides Attached to Iron Complexes 査読あり
Hiroshi Miyazaki, Kenji Usui, Satoshi Fujii
Peptides 2011 2011 224 - 255 2011年
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Construction of Detection System for Amyloid beta Peptide Localization and Aggregation Using Fluorescent and Luminescent Fusion Proteins 査読あり
Kenji Usui, Masayasu Mie, Takashi Andou, Naoki Sugimoto, Hisakazu Mihara, Eiry Kobatake
Peptide Science 2009 2009 111 - 112 2010年
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担当区分:筆頭著者