論文 - 本多 大輔
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Yohei Ishibashi, Hatsumi Goda, Rie Hamaguchi, Keishi Sakaguchi, Takayoshi Sekiguchi, Yuko Ishiwata, Yuji Okita, Seiya Mochinaga, Shingo Ikeuchi, Takahiro Mizobuchi, Yoshitake Takao, Kazuki Mori, Kosuke Tashiro, Nozomu Okino, Daiske Honda, Masahiro Hayashi, Makoto Ito
Communications Biology 4 ( 1 ) 2021年12月
出版者・発行元:Springer Science and Business Media LLC
Abstract
The demand for n-3 long-chain polyunsaturated fatty acids (n-3LC-PUFAs), such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), will exceed their supply in the near future, and a sustainable source of n-3LC-PUFAs is needed. Thraustochytrids are marine protists characterized by anaerobic biosynthesis of DHA via polyunsaturated fatty acid synthase (PUFA-S). Analysis of a homemade draft genome database suggested that Parietichytrium sp. lacks PUFA-S but possesses all fatty acid elongase (ELO) and desaturase (DES) genes required for DHA synthesis. The reverse genetic approach and a tracing experiment using stable isotope-labeled fatty acids revealed that the ELO/DES pathway is the only DHA synthesis pathway in Parietichytrium sp. Disruption of the C20 fatty acid ELO (C20ELO) and ∆4 fatty acid DES (∆4DES) genes with expression of ω3 fatty acid DES in this thraustochytrid allowed the production of EPA and n-3docosapentaenoic acid (n-3DPA), respectively, at the highest level among known microbial sources using fed-batch culture. -
Draft Genome Sequence of Sicyoidochytrium minutum DNA Virus Strain 001 査読あり
Yumi Murakoshi, Takayuki Shimeki, Daiske Honda, Yoshitake Takao
Microbiology Resource Announcements 10 ( 23 ) 2021年6月
出版者・発行元:American Society for Microbiology
Sicyoidochytrium minutum DNA virus strain 001 (SmDNAV 001) is a double-stranded DNA (dsDNA) virus that infects the marine fungoid protist Sicyoidochytrium minutum . We report the draft genome sequence of SmDNAV 001. The 236,345-bp genome contained 358 coding sequences (CDSs) and three tRNA-coding sequences.
DOI: 10.1128/mra.00418-21
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Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. 査読あり 国際共著
Kashiyama, Y., Yokoyama, A., Shiratori, T., Hess, S., Not, F., Bachy, C., Gutierrez-Rodriguez, A., Kawahara, J., Suzaki, T., Nakazawa, M., Ishikawa, T., Maruyama, M., Wang, M., Chen, M., Gong, Y., Seto, K., Kagami, M., Hamamoto, Y., Honda, D., Umetani, T., Shihongi, A., Kayama, M., Matsuda, M., Taira, J., Yabuki, A., Tsuchiya, M., Hirakawa, Y., Kawaguchi, A., Nomura, M., Nakamura, A., Namba, N., Matsumoto, M., Tanaka, T., Yoshino, T., Higuchi, R., Yamamoto, A., Maruyama, T., Yamaguchi, A., Uzuka, A., Miyagishima, S., Tanifuji, G., Kawachi, M., Kinoshita, Y., Tamiaki, H.
The ISME Journal 13 1899 - 1910 2019年2月
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Nutritional intake of Aplanochytrium (Labyrinthulea, Stramenopiles) from living diatoms revealed by culture experiments suggesting the new prey–predator interactions in the grazing food web of the marine ecosystem. 査読あり
Hamamoto, Y., Honda, D.
PLoS ONE 14 ( 1 ) e0208941 2019年1月
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Iwata I, Honda D.
Protist 169 ( 6 ) 978 - 979 2018年12月
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Abe E, Ikeda K, Nutahara E, Hayashi M, Yamashita A, Taguchi R, Doi K, Honda D, Okino N, Ito M.
PLoS ONE 13 ( 8 ) e0203016 - e0203016 2018年8月
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Elemental composition and ultrafine structure of the skeleton in shellbearing protists—A case study of phaeodarians and radiolarians 査読あり
Nakamura, Y., Iwata, I., Hori, R. S., Uchiyama, N., Tuji, A., Fujita, M. J., Honda, D., Ohfuji, H.
Journal of Structural Biology 204 45 - 51 2018年7月
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Nutritional intake by ectoplasmic nets of Schizochytrium aggregatum (Labyrinthulomycetes, Stramenopiles). 査読あり
Iwata, I., Honda, D.
Protist 169 727 - 743 2018年6月
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Possible aplanochytrid (Labyrinthulea) prey detected using 18S metagenetic diet analysis in the key copepod species Calanus sinicus in the coastal waters of the subtropical western North Pacific 査読あり
Hirai, J., Hamamoto, Y., Honda, D., Hidaka, K.
Plankton and Benthos Research 13 ( 2 ) 75 - 82 2018年5月
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DOI: 10.3800/pbr.13.75
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Proposal of Monorhizochytrium globosum gen. nov., comb. nov.(Stramenopiles, Labyrinthulomycetes) for former Thraustochytrium globosum based on morphological features and phylogenetic relationships 査読あり
Doi, K., Honda, D.
Phycological Research 65 ( 3 ) 188 - 201 2017年7月
共著
DOI: 10.1111/pre.12175
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Bothrosome formation in Schizochytrium aggregatum (Labyrinthulomycetes, Stramenopiles) during zoospore settlement 査読あり
Izumi Iwata, Kei Kimura, Yuji Tomaru, Taizo Motomura, Kanae Koike, Kazuhiko Koike, Daiske Honda
Protist 168 ( 2 ) 206 - 219 2017年4月
共著
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Ecological dynamics of two distinct viruses infecting marine eukaryotic decomposer thraustochytrids (Labyrinthulomycetes, Stramenopiles) 査読あり
Yoshitake Takao, Yuji Tomaru, Keizo Nagasaki, Daiske Honda
PLOS ONE 10 ( 7 ) e0133395 2015年7月
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Seasonal dynamics of culturable thraustochytrids (Labyrinthulomycetes, Stramenopiles) in estuarine and coastal waters 査読あり
Ueda, M., Nomura, Y., Doi, K., Nakajima, M., Honda, D.
Aquatic Microbial Ecology 74 187 - 204 2015年3月
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DOI: 10.3354/ame01736
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Novel lysophospholipid acyltransferase PLAT1 of Aurantiochytrium limacinum F26-b responsible for generation of palmitate-docosahexaenoate-phosphatidylcholine and phosphatidylethanolamine. 査読あり
Abe, E., Ikeda, K., Nutahara, E., Hayashi, M., Yamashita A., Taguchi, R., Doi K., Honda D., Okino N., Ito, M.
PLoS ONE 9 ( 8 ) e102377 2014年8月
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Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea. 査読あり
Zhu, D., Tanabe, S., Xie, C., Honda, D., Sun, J., Ai, L.
International Journal of Systematic and Evolutionary Microbiology 64 1712 - 1717 2014年5月
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TLC screening of thraustochytrid strains for squalene production. 査読あり
Nakazawa, A., Kokubun, Y., Matsuura, H., Yonezawa, N., Kose, R., Yoshida, M., Tanabe, Y., Kusuda, E., Thang, D. V., Ueda, M., Honda, D., Mahakhant, A., Kaya, K., Watanabe, M. M.
Journal of Applied Phycology 26 ( 1 ) 29 - 41 2014年2月
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Effect of trace elements on growth of marine eukaryotes, tharaustochytrids 査読あり
Nagano N, Taoka Y, Honda D, Hayashi M.
Journal of Bioscience and Bioengineering 116 ( 3 ) 337 - 339 2013年9月
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Analysis of delta-12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304 査読あり
Matsuda T, Sakaguchi K, Hamaguchi R, Kobayashi T, Abe E, Hama Y, Hayashi M, Honda D, Okita Y, Sugimoto S, Okino N, Ito M
Journal of Lipid Research 53 1210 - 1222 2012年2月
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DOI: 10.1194/jlr.M024935
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Versatile Transformation System That Is Applicable to both Multiple Transgene Expression and Gene Targeting for Thraustochytrids 査読あり
Sakaguchi K, Matsuda T, Kobayashi T, Ohara J, Hamaguchi R, Abe E, Nagano N, Hayashi M, Ueda M, Honda D, Okita Y, Taoka Y, Sugimoto S, Okino N, Ito M
Applied and Environmental Microbiology 78 ( 9 ) 3193 - 3202 2012年2月
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DOI: 10.1128/AEM.07129-11
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On the bio-rearrangement into fully saturated fatty acidscontaining triglyceride in Aurantiochytrium sp. 査読あり
Matsuura H, Nakazawa A, Ueda M, Honda D, Watanabe MM, Kaya K
Procedia Environmental Sciences 15 66 - 72 2012年
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